- CSI的故事背景设在赌城拉斯维加斯，以真人真事改编，讲述刑事警察局的法庭犯罪调查员如何在作案现场取得证据破案的故事。调查员的名言是“死尸会说话”，他们利用指纹、鞋印、子弹壳、血迹、毛发、纤维、尸体伤痕等微小证据，经过仔细的分析研究后，寻得破案的关键。 解开谜团的过程颇有吸引力。
- Russell and Finlay must confront a serial killer case from their past while working in Seattle when a copycat killer suddenly emerges in Las Vegas.
- Under the direction of new boss D.B. Russell, the team tackles cases involving a wild shootout on a Las Vegas tram car, and the death of a deer hunter.
- The CSI team investigates a well-orchestrated bombing at the funeral of Officer Franklin Clark.
- While Ecklie fights for his life after his shooting, Russell's patience begins to wear thin as the team tries to get to his kidnapped granddaughter before she is killed.
- CBS的秋季时间表透露了CSI的最终命运：将推出一个2小时的电视电影作为大结局，元老级角色将回归，包括“葛瑞森”威廉·彼德森、“凯瑟琳”玛格·海根柏格。大结局将于9月27日播出，然后《网络犯罪调查(CSI:Cyber)》第二季接档。
